LOCUS       pcDEST-pcDNA5-miniTurboID-3xFLAG-N-term      7718 bp    DNA   CIRCULAR        04-06-2024
DEFINITION  Gateway Parent Destination Vector, Gateway destination vector with Nterm TurboID-3xFLAG
ACCESSION   V12554
KEYWORDS    
SOURCE      
  ORGANISM  
REFERENCE   1 - 7718 (bases 1 to 7718)
  AUTHORS   Alice Ting/ Anne-Claude
  JOURNAL   
COMMENT     Hala Abdouni created this vector using synthesized human TurboID sequence, replacing V8164 BirA-FLAG with TurboID-3xFLAG-GS2 linker sequence.
FEATURES             Location/Qualifiers
     source          1..7718
     misc_feature    0..0
                     /note=5' linker
                     /gene=
                     /SECDrawas=Region
     misc_feature    0..0
                     /note=3' linker
                     /gene=
                     /SECDrawas=Region
     rep_origin      complement(5963..6551)
                     /note=origin of replication
                     /gene=pBR322
                     /SECDrawas=Region
     polyA_signal    5391..5512)
                     /note=polya tail
                     /gene=SV40
                     /SECDrawas=Region
     polyA_signal    3676..3900)
                     /note=polya tail
                     /gene=BGH
                     /SECDrawas=Region
     CDS             4239..5261
                     /note=selectable marker
                     /gene=Hygromycin Resistance without ATG
                     /SECDrawas=Region
     CDS             3158..3463
                     /note=selectable marker
                     /gene=ccdB
                     /SECDrawas=Region
     CDS             2157..2816
                     /note=selectable marker
                     /gene=Chloramphenicol Resistance
                     /SECDrawas=Region
     CDS             complement(6722..7582)
                     /note=selectable marker
                     /gene=Ampicillin Resistance
                     /SECDrawas=Region
     misc_feature    complement(3504..3628)
                     /note=3' cloning site
                     /gene=attR2
                     /SECDrawas=Region
     misc_feature    1798..1833
                     /note=miscellaneous
                     /gene=GS linker
                     /SECDrawas=Region
     misc_feature    4184..4231
                     /note=miscellaneous
                     /gene=FRT
                     /SECDrawas=Region
     misc_feature    complement(5561..5577)
                     /note=miscellaneous
                     /gene=M13-rev
                     /SECDrawas=Region
     misc_feature    1009..1020
                     /note=miscellaneous
                     /gene=kozak
                     /SECDrawas=Region
     CDS             1834..1899
                     /note=tag
                     /gene=Triple Flag
                     /SECDrawas=Region
     CDS             1015..1791
                     /note=tag
                     /gene=miniTurboID
                     /SECDrawas=Region
     CDS             0..0
                     /organism=
                     /gene=
                     /note=accession: 
                     /note=
                     /SECDrawas=Gene
     promoter        235..614)
                     /note=promoter
                     /gene=CMV enhancer
                     /SECDrawas=Region
     promoter        615..818)
                     /note=promoter
                     /gene=CMV
                     /SECDrawas=Region
     promoter        820..859)
                     /note=promoter
                     /gene=tetO2
                     /SECDrawas=Region
     promoter        complement(5609..5639)
                     /note=promoter
                     /gene=LAC
                     /SECDrawas=Region
     promoter        complement(7583..7687)
                     /note=promoter
                     /gene=Ampicillin
                     /SECDrawas=Region
     promoter        complement(5585..5601)
                     /note=promoter
                     /gene=LacO
                     /SECDrawas=Region
     misc_feature    1924..2048
                     /note=5' cloning site
                     /gene=attR1
                     /SECDrawas=Region

ORIGIN

        1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 
       61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 
      121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 
      181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 
      241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 
      301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 
      361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 
      421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 
      481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 
      541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 
      601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 
      661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 
      721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 
      781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CCCTATCAGT GATAGAGATC 
      841 TCCCTATCAG TGATAGAGAT CGTCGACGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA 
      901 GACGCCATCC ACGCTGTTTT GACCTCCATA GAAGACACCG GGACCGATCC AGCCTCCGGA 
      961 CTCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC CAGCTAGCGC CACCATGGCG 
     1021 ATCCCGCTGC TGAACGCTAA ACAGATTCTG GGACAGCTGG ACGGCGGGAG CGTGGCAGTC 
     1081 CTGCCTGTGG TCGACTCCAC CAATCAGTAC CTGCTGGATC GAATCGGCGA GCTGAAGAGT 
     1141 GGGGATGCTT GCATTGCAGA ATATCAGCAG GCAGGGAGAG GAAGCAGAGG GAGGAAATGG 
     1201 TTCTCTCCTT TTGGAGCTAA CCTGTACCTG AGTATGTTTT GGCGCCTGAA GCGGGGACCA 
     1261 GCAGCAATCG GCCTGGGCCC GGTCATCGGA ATTGTCATGG CAGAAGCGCT GCGAAAGCTG 
     1321 GGAGCAGACA AGGTGCGAGT CAAATGGCCC AATGACCTGT ATCTGCAGGA TAGAAAGCTG 
     1381 GCAGGCATCC TGGTGGAGCT GGCCGGAATA ACAGGCGATG CTGCACAGAT CGTCATTGGC 
     1441 GCCGGGATTA ACGTGGCTAT GAGGCGCGTG GAGGAAAGCG TGGTCAATCA GGGCTGGATC 
     1501 ACACTGCAGG AAGCAGGGAT TAACCTGGAC AGGAATACTC TGGCCGCTAT GCTGATCCGA 
     1561 GAGCTGCGGG CAGCCCTGGA ACTGTTCGAG CAGGAAGGCC TGGCTCCATA TCTGTCACGG 
     1621 TGGGAGAAGC TGGATAACTT CATCAATAGA CCCGTGAAGC TGATCATTGG GGACAAAGAG 
     1681 ATTTTCGGGA TTAGCCGGGG GATTGATAAA CAGGGAGCCC TGCTGCTGGA ACAGGACGGA 
     1741 GTTATCAAAC CCTGGATGGG CGGAGAAATC AGTCTGCGGT CTGCCGAAAA GATGCATGGG 
     1801 GGAGGGGGAG GATCTGGGGG AGGGGGAGGA TCTGACTACA AGGACCACGA CGGTGACTAC 
     1861 AAGGACCACG ACATCGACTA CAAGGACGAC GACGACAAGT TAATTAAGGG CGCGCCACCT 
     1921 AGGACAAGTT TGTACAAAAA AGCTGAACGA GAAACGTAAA ATGATATAAA TATCAATATA 
     1981 TTAAATTAGA TTTTGCATAA AAAACAGACT ACATAATACT GTAAAACACA ACATATCCAG 
     2041 TCACTATGGC GGCCGCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATA 
     2101 ATGTGTGGAT TTTGAGTTAG GATCCGTCGA GATTTTCAGG AGCTAAGGAA GCTAAAATGG 
     2161 AGAAAAAAAT CACTGGATAT ACCACCGTTG ATATATCCCA ATGGCATCGT AAAGAACATT 
     2221 TTGAGGCATT TCAGTCAGTT GCTCAATGTA CCTATAACCA GACCGTTCAG CTGGATATTA 
     2281 CGGCCTTTTT AAAGACCGTA AAGAAAAATA AGCACAAGTT TTATCCGGCC TTTATTCACA 
     2341 TTCTTGCCCG CCTGATGAAT GCTCATCCGG AATTCCGTAT GGCAATGAAA GACGGTGAGC 
     2401 TGGTGATATG GGATAGTGTT CACCCTTGTT ACACCGTTTT CCATGAGCAA ACTGAAACGT 
     2461 TTTCATCGCT CTGGAGTGAA TACCACGACG ATTTCCGGCA GTTTCTACAC ATATATTCGC 
     2521 AAGATGTGGC GTGTTACGGT GAAAACCTGG CCTATTTCCC TAAAGGGTTT ATTGAGAATA 
     2581 TGTTTTTCGT CTCAGCCAAT CCCTGGGTGA GTTTCACCAG TTTTGATTTA AACGTGGCCA 
     2641 ATATGGACAA CTTCTTCGCC CCCGTTTTCA CCATGGGCAA ATATTATACG CAAGGCGACA 
     2701 AGGTGCTGAT GCCGCTGGCG ATTCAGGTTC ATCATGCCGT TTGTGATGGC TTCCATGTCG 
     2761 GCAGAATGCT TAATGAATTA CAACAGTACT GCGATGAGTG GCAGGGCGGG GCGTAAAGAT 
     2821 CTGGATCCGG CTTACTAAAA GCCAGATAAC AGTATGCGTA TTTGCGCGCT GATTTTTGCG 
     2881 GTATAAGAAT ATATACTGAT ATGTATACCC GAAGTATGTC AAAAAGAGGT ATGCTATGAA 
     2941 GCAGCGTATT ACAGTGACAG TTGACAGCGA CAGCTATCAG TTGCTCAAGG CATATATGAT 
     3001 GTCAATATCT CCGGTCTGGT AAGCACAACC ATGCAGAATG AAGCCCGTCG TCTGCGTGCC 
     3061 GAACGCTGGA AAGCGGAAAA TCAGGAAGGG ATGGCTGAGG TCGCCCGGTT TATTGAAATG 
     3121 AACGGCTCTT TTGCTGACGA GAACAGGGGC TGGTGAAATG CAGTTTAAGG TTTACACCTA 
     3181 TAAAAGAGAG AGCCGTTATC GTCTGTTTGT GGATGTACAG AGTGATATTA TTGACACGCC 
     3241 CGGGCGACGG ATGGTGATCC CCCTGGCCAG TGCACGTCTG CTGTCAGATA AAGTCTCCCG 
     3301 TGAACTTTAC CCGGTGGTGC ATATCGGGGA TGAAAGCTGG CGCATGATGA CCACCGATAT 
     3361 GGCCAGTGTG CCGGTCTCCG TTATCGGGGA AGAAGTGGCT GATCTCAGCC ACCGCGAAAA 
     3421 TGACATCAAA AACGCCATTA ACCTGATGTT CTGGGGAATA TAAATGTCAG GCTCCCTTAT 
     3481 ACACAGCCAG TCTGCAGGTC GACCATAGTG ACTGGATATG TTGTGTTTTA CAGTATTATG 
     3541 TAGTCTGTTT TTTATGCAAA ATCTAATTTA ATATATTGAT ATTTATATCA TTTTACGTTT 
     3601 CTCGTTCAGC TTTCTTGTAC AAAGTGGTGT GACTCGAGTC TAGAGGGCCC GTTTAAACCC 
     3661 GCTGATCAGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG 
     3721 TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA 
     3781 TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA 
     3841 GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG GGCTCTATGG 
     3901 CTTCTGAGGC GGAAAGAACC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG 
     3961 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG 
     4021 CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC 
     4081 CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC 
     4141 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAC CTAGAAGTTC CTATTCCGAA 
     4201 GTTCCTATTC TCTAGAAAGT ATAGGAACTT CCTTGGCCAA AAAGCCTGAA CTCACCGCGA 
     4261 CGTCTGTCGA GAAGTTTCTG ATCGAAAAGT TCGACAGCGT CTCCGACCTG ATGCAGCTCT 
     4321 CGGAGGGCGA AGAATCTCGT GCTTTCAGCT TCGATGTAGG AGGGCGTGGA TATGTCCTGC 
     4381 GGGTAAATAG CTGCGCCGAT GGTTTCTACA AAGATCGTTA TGTTTATCGG CACTTTGCAT 
     4441 CGGCCGCGCT CCCGATTCCG GAAGTGCTTG ACATTGGGGA ATTCAGCGAG AGCCTGACCT 
     4501 ATTGCATCTC CCGCCGTGCA CAGGGTGTCA CGTTGCAAGA CCTGCCTGAA ACCGAACTGC 
     4561 CCGCTGTTCT GCAGCCGGTC GCGGAGGCCA TGGATGCGAT CGCTGCGGCC GATCTTAGCC 
     4621 AGACGAGCGG GTTCGGCCCA TTCGGACCGC AAGGAATCGG TCAATACACT ACATGGCGTG 
     4681 ATTTCATATG CGCGATTGCT GATCCCCATG TGTATCACTG GCAAACTGTG ATGGACGACA 
     4741 CCGTCAGTGC GTCCGTCGCG CAGGCTCTCG ATGAGCTGAT GCTTTGGGCC GAGGACTGCC 
     4801 CCGAAGTCCG GCACCTCGTG CACGCGGATT TCGGCTCCAA CAATGTCCTG ACGGACAATG 
     4861 GCCGCATAAC AGCGGTCATT GACTGGAGCG AGGCGATGTT CGGGGATTCC CAATACGAGG 
     4921 TCGCCAACAT CTTCTTCTGG AGGCCGTGGT TGGCTTGTAT GGAGCAGCAG ACGCGCTACT 
     4981 TCGAGCGGAG GCATCCGGAG CTTGCAGGAT CGCCGCGGCT CCGGGCGTAT ATGCTCCGCA 
     5041 TTGGTCTTGA CCAACTCTAT CAGAGCTTGG TTGACGGCAA TTTCGATGAT GCAGCTTGGG 
     5101 CGCAGGGTCG ATGCGACGCA ATCGTCCGAT CCGGAGCCGG GACTGTCGGG CGTACACAAA 
     5161 TCGCCCGCAG AAGCGCGGCC GTCTGGACCG ATGGCTGTGT AGAAGTACTC GCCGATAGTG 
     5221 GAAACCGACG CCCCAGCACT CGTCCGAGGG CAAAGGAATA GCACGTACTA CGAGATTTCG 
     5281 ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT 
     5341 GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA 
     5401 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT 
     5461 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT 
     5521 GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT 
     5581 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG 
     5641 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT 
     5701 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG 
     5761 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 
     5821 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 
     5881 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 
     5941 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 
     6001 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 
     6061 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 
     6121 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 
     6181 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 
     6241 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 
     6301 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 
     6361 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT 
     6421 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 
     6481 AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 
     6541 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 
     6601 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 
     6661 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 
     6721 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 
     6781 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 
     6841 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 
     6901 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 
     6961 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 
     7021 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 
     7081 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 
     7141 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 
     7201 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 
     7261 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 
     7321 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 
     7381 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 
     7441 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 
     7501 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 
     7561 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 
     7621 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 
     7681 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTC  
//
